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Zentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL)

Abteilung Mikrobiologie
Technische Universität München
Weihenstephaner Berg 3
D-85350 Freising

 
 Prof. Dr. Thilo M. Fuchs, Biologist

Telefon: +49-8161-713518
Fax.:      +49-8161-714492
E-Mail: thilo.fuchs @ wzw.tum.de
                                                                                 
      


Research and professional career

 Since 2012

 2006                   
Professor at the TUM

Habilitation

 Since 2002

Scientist at the ZIEL, Department of Microbiology, TU München

1999-2002Project leader at CREATOGEN AG, Augsburg

1998Post-doc scientist in the Helicobacter Vaccine Project at the Max-Plank-Institute for the Biology of Infections, Tübingen

1996-1998Post-doc scientist at the Department of Microbiology, University Würzburg

1994-1995Research stay at the Istituto di Ricerche Immunobiologiche di Siena (R. Rappuoli), Italy

1992-1996Ph. D student at the Theodor-Boveri-Institute in the group of Roy Gross, Department of Microbiology (Prof. W. Goebel), University Würzburg

Fellowships

1997-1998            Scholarship from the Deutsche Forschungsgemeinde (DFG) at the Department of Microbiology, University Würzburg



1. Peer-reviewed articles                

2014

 

Reuter, S., Connor, T. R., Barquist, L., Walker, D., Feltwell, T., Harris, S., Fookes, M., Hall, M. E., Fuchs, T. M., Corander, J., Dufour, M., Ringwood, T., Savin, C., Bouchier, C., Martin, L., Miettinen, M., Shubin, M., Laukkanen-Ninios, R., Sihvonen, L. M., Siitonen, A., Skurnik, M., Falcão, J. P., Fukushima, H., Scholz, H. C., Prentice, M., Wren, B. W., Parkhill, J., Carniel, E., Achtman, M., McNally, A., and N. R. Thomson (2014) Parallel independent evolution of pathogenicity within the genus Yersinia. Proc. Natl. Acad. Sc. U.S.A , accepted. 

Staib, L. and T. M. Fuchs (2014) From food to cell: nutrient exploitation strategies of enteropathogens. Microbiology 160:1020-1039.

Starke, M., and T. M. Fuchs (2014) YmoA negatively controls the expression of insecticidal genes in Yersinia enterocolitica. Mol. Microbiol.92(2):287-301,  doi:10.1111/mmi.12554,

Müller-Herbst, S., Wüstner, S., Krementowski, A., Eder, D., Fuchs, T. M., Held, C., Ehrenreich, A., and S. Scherer (2014) Identification of genes essential for anaerobic growth of Listeria monocytogenes. Microbiology 160:752-765. 

 2013


Starke, M., Richter, M., and T.M. Fuchs (2013) The insecticidal toxin genes of Yersinia enterocolitica are activated by the thermolabile LTTR-like regulator TcaR2 at low temperatures. Mol. Microbiol. 89:596-611

Müller, J., Hense, B.A., Fuchs, T.M., Utz, M., Pötzsche, Ch. (2013) Bet-hedging in stochastically switching environments. J Theor Biol 336:114-157

Neuhaus, K., Satorhely, P., Schauer, K., Scherer, S., and T.M. Fuchs (2013) Acid shock of Listeria monocytogenes at low environmetal temperatures induces prfA, epithelial cell invasion, and lethality towards Caenorhabditis elegans. BMC Genomics 14:285
 

 2012

Fuchs, T.M., Kern, T., Eisenreich, W., Dandekar, T. (2012) Towards a systemic understanding of Listeria monocytogenes metabolism during infection. Frontiers in Microbioal Immunology 3, Article 23:1-12.

Madeo, M., O´Riordan, N., Fuchs, T.M., Utratna, M., Karatzas, K.-A., O´Byrne C. (2012) Thiamine plays a critical role in the acid tolerance of Listeria monocytogenes. FEMS Microbiology Letters 326-137143.

2011

Fuchs, T.M., Eisenreich, W., Heesemann, J., Goebel, W. (2011) Metabolic adaptation of human pathogenic and related non-pathogenic bacteria to extra- and intracellular habitats. FEMS Reviews Microbiology, accepted.

Fuchs, T.M., Brandt, K., Starke, M., Rattei, T. (2011) Shotgun sequencing of Yersinia enterocolitica strain W22703 (biovar 2, serovar O:9): genomic evidence for oscillation between invertebrates and mammals. BMC Genomics 12:168.

Kröger, C., Srikumar, S., Ellwart, J., and T. M. Fuchs (2011) Bistability in myo-inositol utilization by Salmonella enterica serovar Typhimurium. J. Bacteriol. 193, 1427-1435.

Bhowmick, P., Devegowda, D., Ruwandeepika, H. A., Fuchs, T. M., Sreekumar, S., Karunasagar, I., and I. Karunasagar (2011) gcpA (stm1987) is critical for cellulose production and biofilm formation on polystyrene surface by Salmonella enterica serovar Weltevreden in both high and low nutrient medium. Microbial Pathogenesis 50, 114-122.

Srikumar, S., Fuchs, T.M.
 (2011) Ethanolamine utilization contributes to proliferation of Salmonella enterica serovar Typhimurium in food and in nematodes. Appl. Environ. Microbiol. 277, 281-290.


2010

Schauer, K., Geginat, G., Liang, C., Goebel, W., Dandekar, T., Fuch, T.M. (2010) Deciphering the intracellular metabolism of Listeria monocytogenes by mutant screening and modelling. BMC Genomics 11:573.

Dolowschiak, T., Chassin, C., Mkaddem, S. B., Fuchs, T.M., Weiss,
S., Vandewalle, A., Hornef, M.W. (2010) Potentiation of epithelial innate host responses by intercellular communication. PLoS Pathogens 6:e1001194.

Spanier, B., Starke, M., Higel, F., Scherer, S., Fuchs, T.M. (2010). Yersinia enterocolitica infection and tcaA-dependent killing of Caenorhabditis elegans. Appl. Environ. Microbiol. 76, 6277-6285.

Trcek, J., Fuchs, T. M., Trülzsch, K.  (2010) Analysis of Y. enterocolitica invasin expression in vitro and in vivo using a novel luxCDABE reporter system. Microbiology 156, 2734 - 2745.

Schär, J., Stoll, R., Schauer, K., Loeffler, D.I.M., Eylert, E., Joseph, B., Eisenreich W,  Fuchs, T. M., Goebel, W.  (2010) Pyruvate carboxylase plays a crucial role in carbon metabolism of extra- and intracellularly replicating Listeria monocytogenes. J. Bacteriol.192, 1774-1784.

Kröger, C., Stolz, J., Fuchs, T.M. (2010) Myo-Inositol transport by Salmonella enterica serovar Typhimurium. Microbiology 156, 128-138.

2006 - 2009

Schauer, K., Stolz, J., Scherer, S., Fuchs, T.M. (2009) Both thiamine uptake and biosynthesis of thiamine precursors are required for intracellular replication of Listeria monocytogenes. J. Bacteriol. 191, 2218-2227.

Kröger, C., Fuchs, T.M. (2009) Characterization of the myo-inositol utilization
island of Salmonella typhimurium. J. Bacteriol. 191, 545-554.

Fuchs, T. M., Bresolin, G., Marcinowski, L., Schachtner, J., Scherer, S. (2008) Insecticidal genes of Yersinia spp.: taxonomical distribution, contribution to toxicity towards Manduca sexta and Galleria mellonella, and evolution. BMC Microbiology 8:214.

Heermann, R., Fuchs, T.M. (2008) Comparative analysis of the Photorhabdus luminescens and the Yersinia enterocolitica genomes: uncovering candidate genes involved in insect pathogenicity. BMC Genomics 9:40.
 
Bresolin, G., Trcek, J., Scherer, S., Fuchs, T.M.  (2008) Presence of a functional
flagellar cluster Flag-2 and low-temperature expression of flagellar genes in Yersinia enterocolitica W22703. Microbiology 154, 196-206.

Klumpp, J., Fuchs, T.M.  (2007) Identification of novel genes in genomic islands that contribute to Salmonella typhimurium replication in macrophages. Microbiology 153, 1207-1220.

Bresolin, G., Morgan, A., Ilgen, D., Scherer, S., Fuchs, T.M.  (2006) Low temperature-induced insecticidal activity of Yersinia enterocolitica. Mol. Microbiol. 59, 503-512. 

Joseph, B., Przybilla, K., Stühler, C., Schauer, K., Slaghius, J., Fuchs, T. M., Goebel, W. (2006) Identification of Listeria monocytogenes genes contributing to intracellular replication by expression profiling and mutant screening. J. Bacteriol. 188, 556-568.

Bresolin, G., Neuhaus, K., Scherer, S., Fuchs, T.M.  (2006) Transcriptional analysis of long-term adaptation of Yersinia enterocolitica to low temperature growth. J. Bacteriol. 188, 2945-2958.

 2002 - 2005

Middendorf, B, Stübs, D., Guiso, N., Deppisch, H., Gross, R., Fuchs, T.M. (2005) Phg, a novel member of the autotransporter family present in Bordetella species. Microbiological Research, 160, 329-336.

Fuchs, T. M., Klumpp, J., Przybilla, K. (2005) Insertion-duplication mutagenesis (IDM) of Salmonella enterica and related species using a novel thermosensitive vector. Plasmid 55, 39-49.

Stübs, D., Fuchs, T.M., Schneider, B., Bosserhoff, A., Gross, R. (2005) Identification and regulation of cold inducible factors of Bordetella bronchiseptica. Microbiology, 151, 1911-1917.

Knuth, K., Niesalla, H., Hueck, C. J., Fuchs, T.M. (2004) Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol. Microbiol., 51, 1729-1744

König, J., Bock, A., Perraud, A.-L., Fuchs, T. M., Beier, D., Gross, R. (2002) Regulatory factors of Bordetella pertussis affecting virulence gene expression. J. Mol. Microbiol. Biotechnol. 4, 197-203.

1992 - 2000

Fuchs, T.M., Schneider, B., Krumbach, K., Eggeling, L., Gross, R. (2000) Characterization of a Bordetella pertussis diaminopimelate (DAP) biosynthesis locus identifies dapC, a novel gene coding for an N-succinyl-L,L-DAP aminotransferase. J. Bacteriol. 182:3626-3631.

Fuchs, T. M. (1998) Molecular mechanisms of bacterial pathogenicity.
 Naturwissenschaften 85, 99-108.

Fuchs, T.M., Deppisch, H., Scarlato, V., Gross, R. (1996) A new gene locus of Bordetella pertussis defines a novel family of prokaryotic transcriptional accessory proteins. J. Bacteriol. 178, 4445-4452.

Beier, D., Fuchs, T.M., Graeff-Wohlleben, H., Gross R. (1996) Signal transduction and virulence regulation in Bordetella pertussis. Microbiologia SEM 12:185-196.

Beier, D., Schwarz, B., Fuchs, T.M., Gross, R. (1995). In vivo characterization of the unorthodox BvgS two-component sensor protein of Bordetella pertussis. J. Mol. Biol. 248, 596-610.

Carbonetti, N.H., Fuchs, T.M., Patamawenu, A. A., Irish, T. J., Deppisch, H., Gross, R. (1994) Effect of mutations causing overexpression of RNA polymerase alpha subunit on regulation of virulence factors in Bordetella pertussis. J. Bacteriol. 176, 7267-7273.

Fuchs, T.M., Beier, D., Beier, H. (1992). The tRNA Tyr multigene family of Nicotiana rustica: genome organization, sequence analysis and expression in vitro. Plant Molecular Biology 20, 869-878.



2. Others

Fuchs, T.M., Neuhaus, K., Scherer S. (2012) Life at cold temperatures. The Procaryotes. Invited review, accepted.

Fuchs, T.M., Scherer S. (2010) Yersinia-effiziente Killer. Labor&More 2.10, 66-69.

Schoen, C., Stritzker, J., Fuchs, T.M., Weibel, S., Gentschev, I., Szalay, A.A., Goebel, W. (2008) Use of intracellular bacteria for the development of tool for tumor therapy and the detection of novel antibacterial targets. Patho-Biotechnology (eds.: Sleator R. and C.Hill).

Hain, T., Chatterjee, S. S.,Ghai, R, Kuenne, C. T., Billion, A., Steinweg, C, Domann, E., Kärst, U., Jänsch, L., Wehland, J., Eisenreich, W., Bacher, A., Joseph, B., Schär, J., Kreft, J., Klumpp, J., Loessner, M. J., Dorscht, J., Neuhaus, K., Fuchs, T. M., Scherer, S., Doumith, M., Jacquet, C., Martin, P., Cossart, P., Glaser, P., Buchrieser, C., Goebel, W.,  Chakraborty, T. (2007) Pathogenomics of Listeria ssp. Int. J. Med. Microbiol., 2007.

Bresolin, G., Scherer, S., Fuchs, T.M. (2006) Yersinia als Insektenkiller. Biospektrum 02, 171-172.

Fuchs, T. M. (2005) Reduzierte Genome und das Konzept eines minimalen Gensets. GenomXpress 01, 22-24.

König, J., Bock, A., Perraud, A.-L., Fuch, T.M., Beier, D., Gross, R. (2003) Virulenz gene regulation in Bordetella pertussi. In: Regulatory Networks in Prokaryotes. Eds: Dürre P and Friedrich B.Horizon,  Scientific Press, pp.27-33.

Fuchs, T.M., Gross, R. (2001) Die molekularen Talente des Keuchhustenerregers. Biologie in unserer Zeit 1, 10-20.

Fuchs, T.M., Gross, R. (1996) The virulence factors of Bordetella pertussis and their regulation. Alpe Adria Microbiology Journal 5, 179-190.

Beier, D., Schwarz, B., Deppisch, H., Fuchs, T.M., Perraud, A.-L., Gross, R. (1996) Structure-function relationship in BvgS-the master regulon of virulence in Bordetella pertussis. In: Bacterial protein toxins (Frandsen et al., eds), Gustav Fischer Verlag, Stuttgart, pp:1-12.

Gross, R., Fuchs,T.M., Deppisch, H., Carbonetti, N.H. (1994) Virulence regulation in Bordetella pertussis. In: Molecular Mechanisms of Bacterial Virulence (Kado, C.I. and Crosa, J., eds), Kluwer Academic Publishers, New York, pp:511-524.

Gross, R., Beier, D., Deppisch, H., Fuchs, T.M., Graeff, H., Schipper, H. (1994) Umweltbedingte Modulation der Virulenz von Bordetella pertussis. In: Modulation der Virulenz von Krankheitserregern (Hacker, J. and Heesemann, J., eds) Socio Medico Verlag Gräfelfing, pp:35-51.

3. Minireviews

Fuchs, T.M. (2011) Salmonellen: die dunkle Seite des Salats? Biospektrum 07.11:781.

Fuchs, T.M. (2011) Mikrobielle Endokrinologie: Infektionskrankheiten und Stress. Biospektrum 05.11:549.

Fuchs, T.M. (2011) Salmonella - From Genome to Function. Biospektrum 05.11:596.

Fuchs, T.M. (2010) Meister im Manipulieren: Listeria monocytogenes. Biospektrum 01.10:41. 

Fuchs, T.M. (2010) Ein Virulenzregulator der Yersinien mit Thermometer. Biospektrum 01.10:47.


Aktuelles

Paper
Permanent colonization of creek sediments, creek water and limnic water plants by four Listeria species in low population densities (November 2016)

Paper
Comparative Bioinformatics and Experimental Analysis of the Intergenic Regulatory Regions of Bacillus cereus hbl and nhe Enterotoxin Operons and the Impact of CodY on Virulence Heterogeneity (Mai 2016)

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Draft genome sequences of three European laboratory derivatives from Enterohemorrhagic Escherichia coli O1.57:H7 Strain EDL933, including two plasmids. (März 2016)

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Regulation of fucose and 1,2-propanediol utilization by Salmonella enterica serovar Typhimurium (März 2016)

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Acidified nitrite inhibits proliferation of Listeria monocytogenes - Transcriptional analysis of a preservation method (März 2016)

Berufung Expertenrat
Prof. Dr. Siegfried Scherer wurde in den Expertenrat für Lebensmittelsicherheit des Bayerischen Staatsministeriums für Umwelt und Verbraucherschutz berufen.

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Translatomics combined with transcriptomics and proteomics reveals novel functional, recently evolved orphan genes in Escherichia coli O157:H7 (EHEC)

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A sensitive and robust method for direct determination of lipolytic activity in natural milk environment (März 2016, online first)

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Spoilage of vacuum-packed beef by the yeast Kazachstania psychrophila (Jan 2016)

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VisExpress: Visual exploration of differential gene Expression data (Dez. 2015)

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Evidence for the recent origin of a bacterial protein-coding, overlapping orphan gene by evolutionary overprinting (Dez. 2015).

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Pathogen-nematode interactioni: Nitrogen supply of Listeria monocytogenes during growth in Caenorhabditis elegans (Dez 2015)

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Massive horizontal gene transfer, strictly vertical inheritance and ancient duplications differentially shape the evolution of Bacillus cereus enterotoxin operons hbl, cytK and nhe (Nov. 2015)

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The Mutation Glu151Asp in the B-Component of the Bacillus cereus Non-Hemolytic Enterotoxin (Nhe) Leads to a Diverging Reactivity in Antibody-Based Detection Systems (Nov. 2015)

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Ces locus embedded proteins control the non-ribosomal synthesis of the cereulide toxin in emetic Bacillus cereus on multiple Levels (Oktober 2015)

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Biodiversity of refrigerated raw milk microbiota and their enzymatic spoilage potential (Sept.2015)

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Isolation and characterisation of a heat-resistant peptidase from Pseudomonas panacis withstanding general UHT processes (Sept. 2015)

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Depsipeptide intermediates interrogate proposed biosynthesis of Cereulide, the Emetic Toxin of Bacillus cereus (Mai 2015)

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From genome to toxicity: a combinatory approach Highlights the complexity of enterotoxin production in Bacillus cereus (Juni 2015)

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Chemodiversity of cereulide, the emetic Toxin of Bacillus cereus (2015)

Paper
Comparison of strand-specific transcriptomes of enterohemorrhagic Escherichia coli O157:H7 EDL933 (EHEC) under eleven different environmental conditions including radish sprouts and cattle feces (Juni 2014)

AiF 17506 Schlussbericht
Enteropathogene Bacillus cereus in Lebensmitteln: Identifizierung und Risikoabschätzung

AiF 16908 N Schlussbericht
Mikrobiologische Sicherheit von Rohwurstprodukten - Wirkung von Nitrit und Pflanzenextrakten auf enterohämorrhagische Escherichia coli und Salmonella spp.

AiF 16588 N Schlussbericht
Hitzestabile mikrobielle Enzyme in Rohstoffen zur Milchverarbeitung – Qualitätssicherung, Entwicklung eines Testsystems und technologische Optionen

AiF 16845 N Kurzbericht
Entwicklung einer routinetauglichen Quantifizierungsmethode von Cereulid aus B. cereus und Studien zur Bildung und Stabilität des Toxins in Lebensmitteln

Forschungsbericht
Emetische Toxinproduktion von Bacillus cereus in ausgewählten Lebensmitteln: Mechanismen und Präventionsmöglichkeiten