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Analysis of Pseudomonas and heat-resistant peptidases in raw milk

Group leader until 2021: Dr. Genia Lücking


Heat-resistant bacterial peptidases in raw milk are often associated with premature spoilage of milk and pose a problem regarding the quality of long-life milk products. Studies have determined Pseudomonas as the most common genus in raw milk possessing the highest proteolytic activity.

Our current research deals with the regulation of peptidase production and the development of a detection method for milk-relevant Pseudomonas species. Another project focuses on the identification of entry routes for Pseudomonas into raw milk including the analysis of farm-specific factors.


Publications

Maier C, Hofmann K, Huptas C, Scherer S, Wenning M, Lücking G (2021) Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR. Applied Microbiology and Biotechnology. Doi10.1007/s00253-021-11109-0

Maier C, Huptas C, von Neubeck M, Scherer S, Wenning M, Lücking G (2020) Genetic organization of the aprX-lipA2 operon affects the proteolyticpotential of Pseudomonas species in milk. Frontiers in Microbiology, Vol 11, Article 1190. Doi 10.3389/fmicb.2020.01190

von Neubeck M, Huptas C, Glück C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M (2016)
Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanen-sis sp. nov., isolated from raw cow’s milk. Int J Syst Evol Microbiol; 66:1163–1173

Stoeckel M, Lidolt M, Stressler T, Fischer L, Wenning M, Hinrichs J (2016) Heat stability of indigenous milk plasmin and peptidases from Pseudomonas: a challenge in the production of ultra-high temperat-ure milk products. Int Dairy J; 61:250-261 

Glück C, Rentschler E, Krewinkel M, Merz M, von Neubeck M, Wenning M, Scherer S, Stoeckel M, Hinrichs J, Stressler T, Fischer L (2016) Thermostability of peptidases secreted by raw milk associated microorganisms. Int Dairy Journal; 56:186-19

Stoeckel M, Friedl N, Achberger V, Lidolt M, Glück C, Krewinkel M, Stressler T, von Neubeck M, Wenning M, Scherer S, Fischer L, Hinrichs J (2016) Growth of Pseudomonas weihenstephanensis, Pseudomonas proteolytica and Pseudomonas sp. in raw milk: Impact of residual heat-stable enzyme activity on stability of UHT milk during shelf-life. Int Dairy Journal; 59:20-28

von Neubeck M, Huptas C, Glück C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M (2016) Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow´s milk. Int J System Evol Microbiol; 66:1163-1173

Baur C, Krewinkel M, Kutzli I, Kranz B, von Neubeck M, Huptas C, Wenning M, Scherer S, Stoeckel M, Hinrichs J, Stressler T, Fischer L (2015) Isolation and characterisation of a heat-restistant peptidase from Pseudomonas panacis withstanding General UHT processes. Int Dairy J; 49:46-55

Baur C, Krewinkel M, Kutzli I, Kranz B, von Neubeck M, Scherer S, Stoeckel M, Hinrichs J, Stressler T, Fischer L (2015) Quantification of the proteolytic and lipolytic activity of microorganisms isolated from raw milk. Int Dairy J; 49:23-29



News

Paper
High Counts of thermophilic spore Formers in dairy powders originate from persisting strains in processing lines (2020)

Paper
Dynamically evolving novel overlapping gene as a factor in the SARS-CoV-2 pandemic (Okt. 2020)

Paper
Are antisense proteins in prokaryotes functional (2020)

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Complementary use of cultivation and high-throughput amplicon sequencing reveals high biodiversity within raw milk microbiota (2020)

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Recommendations for bacterial ribosome profiling experiments based on bioinformatics evaluation of published data (2020)

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OLGenie: Estimating natural selection to predict functional overlapping genes (2020)

Paper
Genetic Organization of the aprX-lipA2 Operon Affects the Proteolytic Potential of Pseudomonas Species in Milk (2020)

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Brevilactibacter flavus gen.nov., sp. nov., a novel bacterium of the family Propionibactericeae isolated form raw milk and dairy products and reclassification of Probioniciclava sinopodophylli as Brevilactibacter sinopodophylli comb. nov. (2020)

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A novel pH-regulated, unusual 603 bp overlapping protein coding gene pop is encoded antisense to ompA in Escherichia coli O157:H7 (EHEC). (2020)

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Pseudomonas haemolytica sp. nov., isolated from raw milk and skimmed milk concentrate (2020)

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Pseudomonas saxonica sp.nov. isolated from raw milk and skimmed milk concentrate. (2020)