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Zentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL)

Abteilung Mikrobiologie
Technische Universität München
Weihenstephaner Berg 3
D-85350 Freising


Analysis of Pseudomonas and heat-resistant peptidases in raw milk

Group leader: Dr. Genia Lücking

Inge Celler, Technician
Charon Fuhrmann, Technician
Katharina Jentsch, MSc, Doktorandin
Christopher Maier, MSc, Doktorand

Heat-resistant bacterial peptidases in raw milk are often associated with premature spoilage of milk and pose a problem regarding the quality of long-life milk products. Studies have determined Pseudomonas as the most common genus in raw milk possessing the highest proteolytic activity.

Our current research deals with the regulation of peptidase production and the development of a detection method for milk-relevant Pseudomonas species. Another project focuses on the identification of entry routes for Pseudomonas into raw milk including the analysis of farm-specific factors.


Publications

von Neubeck M, Huptas C, Glück C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M (2016) Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanen-sis sp. nov., isolated from raw cow’s milk. Int J Syst Evol Microbiol; 66:1163–1173

Stoeckel M, Lidolt M, Stressler T, Fischer L, Wenning M, Hinrichs J (2016) Heat stability of indigenous milk plasmin and peptidases from Pseudomonas: a challenge in the production of ultra-high temperat-ure milk products. Int Dairy J; 61:250-261 

Glück C, Rentschler E, Krewinkel M, Merz M, von Neubeck M, Wenning M, Scherer S, Stoeckel M, Hinrichs J, Stressler T, Fischer L (2016) Thermostability of peptidases secreted by raw milk associated microorganisms. Int Dairy Journal; 56:186-19

Stoeckel M, Friedl N, Achberger V, Lidolt M, Glück C, Krewinkel M, Stressler T, von Neubeck M, Wenning M, Scherer S, Fischer L, Hinrichs J (2016) Growth of Pseudomonas weihenstephanensis, Pseudomonas proteolytica and Pseudomonas sp. in raw milk: Impact of residual heat-stable enzyme activity on stability of UHT milk during shelf-life. Int Dairy Journal; 59:20-28

von Neubeck M, Huptas C, Glück C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M (2016) Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow´s milk. Int J System Evol Microbiol; 66:1163-1173

Baur C, Krewinkel M, Kutzli I, Kranz B, von Neubeck M, Huptas C, Wenning M, Scherer S, Stoeckel M, Hinrichs J, Stressler T, Fischer L (2015) Isolation and characterisation of a heat-restistant peptidase from Pseudomonas panacis withstanding General UHT processes. Int Dairy J; 49:46-55

Baur C, Krewinkel M, Kutzli I, Kranz B, von Neubeck M, Scherer S, Stoeckel M, Hinrichs J, Stressler T, Fischer L (2015) Quantification of the proteolytic and lipolytic activity of microorganisms isolated from raw milk. Int Dairy J; 49:23-29



News

Paper
Optimality in the standard gentic code is robust with respect to comparison code sets (2019)

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Accurate quantification of thermophilic spores in dairy powders (2019)

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Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk (2017)

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Resistance of thermophilic spore formers isolated from milk and whey products towards cleaning-in-place conditions: Influence of pH, temperature and milk residues (2019)

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Proposal of Lysobacter pythonis sp. nov. isolated from royal pythons (Python regius) (2019)

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The novel EHEC gene asa overlaps the TEGT transporter gene in antisense and is regulated by NaCl and growth Phase (Dec 2018)

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The small RNA RssR regulates myo-inositol degradation by Salmonella enterica (Dec.2018)

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Activity of a Holin-Endolysin System in the Insecticidal Pathogenicity Island of Yersinia enterocolitica (2018)

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Reprogramming Human Siderocalin to Neutralize Petrobactin, the Essential Iron Scavenger of Anthrax Bacillus (2018)

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Insecticidal Toxicity of Yersinia frederiksenii Involves the Novel Enterotoxin YacT (2018)

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Dysfunction, Disease, and the Limits of Selection (2018)

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Dynamic Proteome Alteration and Functional Modulation of Human Saliva Induced by Dietary Chemosensory Stimuli (2018)

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Genome sequence of the novel virulent bacteriophage PMBT14 with lytic avtivity against Pseudomonas fluorescens DSM 50090 (2018)

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The Novel Anaerobiosis-Responsive Overlapping Antisense to the Annotated Gene ECs2385 of Escherichia coli O157:H7 Sakai (Mai 2018)

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Thermal treatment of skim milk concentrates in a novel shear-heating device: Reduction of thermophilic spores and physical properties (Febr. 2018)

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A novel short L-arginine responsive protein-coding gene (laoB) antiparallel overlapping to a CadC-like transcriptional regulator in Escherichia coli O157:H7 Sakai originated by overprinting (2018)

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Thermal resistance of vegetative thermophilic spore forming bacilli in skim milk isolated from dairy Environments (2017)

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Comparison between Listeria sensu stricto and Listeria sensu lato strains identifies novel determinants involved in infection (2017)

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Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq-ryhB encodes the regulatory RNA RyhB and a Peptide, RyhP. (2017)

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Complete circular genome sequence and temperature independent adaptation to Anaerobiosis of Listeria weihenstephanensis DSM 24698 (2017)

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Discovery of numerous novel small genes in the intergenic regions of the Escherichia coli O157:H7 Sakai Genome (2017)

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Spoilage of Microfiltered and Pasteurized Extended Shelf Life Milk Is Mainly Induced by Psychrotolerant Spore-Forming Bacteria that often Originate from Recontamination (2017)

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Transcriptional and translational regulation by RNA thermometers, riboswitches and the sRNA DsrA in Escherichia coli O157:H7 Sakai under combined cold and osmotic stress adaptation. (2016)

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Comparative Bioinformatics and Experimental Analysis of the Intergenic Regulatory Regions of Bacillus cereus hbl and nhe Enterotoxin Operons and the Impact of CodY on Virulence Heterogeneity (Mai 2016)

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Acidified nitrite inhibits proliferation of Listeria monocytogenes - Transcriptional analysis of a preservation method (März 2016)

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Translatomics combined with transcriptomics and proteomics reveals novel functional, recently evolved orphan genes in Escherichia coli O157:H7 (EHEC)

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Evidence for the recent origin of a bacterial protein-coding, overlapping orphan gene by evolutionary overprinting (Dez. 2015)

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Massive horizontal gene transfer, strictly vertical inheritance and ancient duplications differentially shape the evolution of Bacillus cereus enterotoxin operons hbl, cytK and nhe (Nov. 2015)