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Zentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL)

Abteilung Mikrobiologie
Technische Universität München
Weihenstephaner Berg 3
D-85350 Freising


Analysis of Pseudomonas and heat-resistant peptidases in raw milk

Group leader: Dr. Genia Lücking

Inge Celler, Technician
Charon Fuhrmann, Technician
Katharina Jentsch, MSc, Doktorandin
Christopher Maier, MSc, Doktorand

Heat-resistant bacterial peptidases in raw milk are often associated with premature spoilage of milk and pose a problem regarding the quality of long-life milk products. Studies have determined Pseudomonas as the most common genus in raw milk possessing the highest proteolytic activity.

Our current research deals with the regulation of peptidase production and the development of a detection method for milk-relevant Pseudomonas species. Another project focuses on the identification of entry routes for Pseudomonas into raw milk including the analysis of farm-specific factors.


Publications

von Neubeck M, Huptas C, Glück C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M (2016) Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanen-sis sp. nov., isolated from raw cow’s milk. Int J Syst Evol Microbiol; 66:1163–1173

Stoeckel M, Lidolt M, Stressler T, Fischer L, Wenning M, Hinrichs J (2016) Heat stability of indigenous milk plasmin and peptidases from Pseudomonas: a challenge in the production of ultra-high temperat-ure milk products. Int Dairy J; 61:250-261 

Glück C, Rentschler E, Krewinkel M, Merz M, von Neubeck M, Wenning M, Scherer S, Stoeckel M, Hinrichs J, Stressler T, Fischer L (2016) Thermostability of peptidases secreted by raw milk associated microorganisms. Int Dairy Journal; 56:186-19

Stoeckel M, Friedl N, Achberger V, Lidolt M, Glück C, Krewinkel M, Stressler T, von Neubeck M, Wenning M, Scherer S, Fischer L, Hinrichs J (2016) Growth of Pseudomonas weihenstephanensis, Pseudomonas proteolytica and Pseudomonas sp. in raw milk: Impact of residual heat-stable enzyme activity on stability of UHT milk during shelf-life. Int Dairy Journal; 59:20-28

von Neubeck M, Huptas C, Glück C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning M (2016) Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow´s milk. Int J System Evol Microbiol; 66:1163-1173

Baur C, Krewinkel M, Kutzli I, Kranz B, von Neubeck M, Huptas C, Wenning M, Scherer S, Stoeckel M, Hinrichs J, Stressler T, Fischer L (2015) Isolation and characterisation of a heat-restistant peptidase from Pseudomonas panacis withstanding General UHT processes. Int Dairy J; 49:46-55

Baur C, Krewinkel M, Kutzli I, Kranz B, von Neubeck M, Scherer S, Stoeckel M, Hinrichs J, Stressler T, Fischer L (2015) Quantification of the proteolytic and lipolytic activity of microorganisms isolated from raw milk. Int Dairy J; 49:23-29



News

Paper
Complementary use of cultivation and high-throughput amplicon sequencing reveals high biodiversity within raw milk microbiota (2020)

Paper
Recommendations for bacterial ribosome profiling experiments based on bioinformatics evaluation of published data (2020)

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OLGenie: Estimating natural selection to predict functional overlapping genes (2020)

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Genetic Organization of the aprX-lipA2 Operon Affects the Proteolytic Potential of Pseudomonas Species in Milk (2020)

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Brevilactibacter flavus gen.nov., sp. nov., a novel bacterium of the family Propionibactericeae isolated form raw milk and dairy products and reclassification of Probioniciclava sinopodophylli as Brevilactibacter sinopodophylli comb. nov. (2020)

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A novel pH-regulated, unusual 603 bp overlapping protein coding gene pop is encoded antisense to ompA in Escherichia coli O157:H7 (EHEC). (2020)

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Pseudomonas haemolytica sp. nov., isolated from raw milk and skimmed milk concentrate (2020)

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Pseudomonas saxonica sp.nov. isolated from raw milk and skimmed milk concentrate. (2020)

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Thermally induced milk fouling: Survival of thermophilic spore Formers and potential of contamination (Dez 2019)

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Optimality in the standard gentic code is robust with respect to comparison code sets (2019)

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Accurate quantification of thermophilic spores in dairy powders (2019)

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Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk (2017)

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Resistance of thermophilic spore formers isolated from milk and whey products towards cleaning-in-place conditions: Influence of pH, temperature and milk residues (2019)

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Proposal of Lysobacter pythonis sp. nov. isolated from royal pythons (Python regius) (2019)

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The novel EHEC gene asa overlaps the TEGT transporter gene in antisense and is regulated by NaCl and growth Phase (Dec 2018)

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Reprogramming Human Siderocalin to Neutralize Petrobactin, the Essential Iron Scavenger of Anthrax Bacillus (2018)

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Dysfunction, Disease, and the Limits of Selection (2018)

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The Novel Anaerobiosis-Responsive Overlapping Antisense to the Annotated Gene ECs2385 of Escherichia coli O157:H7 Sakai (Mai 2018)

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A novel short L-arginine responsive protein-coding gene (laoB) antiparallel overlapping to a CadC-like transcriptional regulator in Escherichia coli O157:H7 Sakai originated by overprinting (2018)